Mar 23, Arrabidaea chica (HBK) Verlot, Bignoniaceae, is a scrambling shrub native to tropical America, more particularly in the Amazon basin where it. Arrabidaea chica (Humb. & Bonpl.) Verl. Show All Show Tabs cricketvine. General Information. Symbol: ARCH5. Group: Dicot. Family: Bignoniaceae. Duration. A new flavone, 6,7,3′,4′-tetrahydroxymethoxyflavone, named carajuflavone, was isolated from the leaves of the Brazilian plant Arrabidaea chica f. cuprea.
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Effect of Arrabidaea chica extracts on the Ehrlich solid tumor development. Ribeiro I ; Thalita C. Telles II ; Vany P. Carvalho VI arrabidea Marilia M. The aim of this study was to investigate the effect of Arrabidaea chica Humb. The phytochemical analysis of the extracts indicated different classes of secondary metabolites like as anthocyanidins, flavonoids, tannins and saponins.
BioMed Research International
The antitumor activity presented by the AE is possibly related to an anti-inflammatory activity. None of the extracts produced toxic effects in animals. In conclusion, the ethanol and aqueous extracts of A. These effects appear to be related to different mechanisms of action for each extract.
arrabidaea chica leaf/stem extract
This study demonstrates the potential of A. Arrabidaea chica, Bignoniaceae, flavonols, Ehrlich tumor, antitumor activity. Cancer is one of the leading arranidaea of mortality worldwide. Many of the chemotherapeutic agents used in the treatment of cancer injure rapidly dividing normal cells presenting substantial side effects. Therefore, the quest for effective anticancer drugs is an active research field.
The plant kingdom is a potential source of chemicals with antitumor and cytotoxic activities Kim et al. The leaves of the plant have been traditionally used by Brazilians Indians as dye in ritual body painting, to protect the skin against the sunlight and to arraibdaea insects Chapman et al, Very little is known about the chemical constitution of the leaves Barbosa et al.
Earlier studies of A. Later, a flavone Takemura, and three anthocyanidins Zorn et al. More recently the main classes of secondary metabolites in the ethanol extract was detected: Despite the wide use very little is known about the pharmacological properties of its extracts Barbosa et al. The anti-inflammatory effect was already demonstrated in vivo Oliveira et al. Also, healing and antioxidant activities were reported, possibly related to the presence of anthocyanidins Jorge et al.
Therefore, based on the empirical ethnopharmacological use of Arrabidaea chica crude extract as an antitumor agent associated with the strong cytotoxic activity in vitro Ribeiro et al.
Leaves of Arrabidaea chica Humb. The plant material was identified in Campinas-SP, Brazil, where a voucher specimen is deposited and chicz under the number UEC The leaves of A.
This extract was fractionated according to Matos Briefly, the pH was basified with ammonium hydroxide pH A arrabidea of n -hexane and ethyl acetate 1: After separation, hydrochloric acid pH 1. The pH was basified pH 7. The ethanol extraction was performed as previously described Leite et al.
Briefly, the ethanol extract EE was arrabidaaea by maceration of dry leaves g for exhaustive percolation with ethanol at room temperature. After chida, the solvent ethanol was evaporated under reduced pressure to yield 1. Chemical tests to detect the main classes of secondary metabolites were carried out in aqueous extract AE using arrabidaaea specific color reactions Matos, It was delivered at a flow rate of 1.
The retention time of kaempferol was found to be Analysis was performed at room temperature. The cells were maintained in vivo in Swiss albino mice by intraperitoneal transplantation.
EAC cells aspirated from the peritoneal cavity of mice were washed with saline and injected subcutaneously to develop solid tumor. The selection of the dose was based on the work of Queiroz et al.
Tumor growth was evaluated by measuring the inoculated footpad thickness with a graduate micrometer Mitutoyo, modelgraduation 0. This procedure was done at every 48 h. On the 10 th day of treatment, i. The four micrometers sections were stained in hematoxilin and eosin for histological examination. Blood samples were collected by retro-orbital puncture for hematological evaluation before the euthanasia.
Effect of Arrabidaea chica extracts on the Ehrlich solid tumor development
Bands were scanned and measured using the software Celm SE Analysis of the mononuclear arrabixaea subpopulations in blood and tumor tissue by flow cytometry. In this protocol the number of five types of mononuclear leukocytes on the blood and tumor tissue were evaluated: The tumor tissue was squashed in RPMI 5 mL media and filtrated on stainless steel gaze to obtain a single cell suspension.
The cell suspension was kept on ice for 5 min and washed twice in RPMI The protocols, antibodies and reagents employed were as per the manufacturer’s recommendations Sigma. All data were checked for normality. Kruskal-Wallis or Mann-Whitney U tests were used when data were not normally distributed.
In order to evaluate if Arrabidaea chica Humb.
Measures of mice paws showed a continuous tumor growth in all experimental groups. Both groups treated with AE and EE showed similar patterns of tumor development – a steady growth without the occurrence of peaks during the experimental period, while the control group presented a peak seven days after the inoculation Table 1.
In 12 th arrabidaeea, the EE group presented a smaller tumor development 2. The growth curve of the Ehrlich tumor presents an exponential behavior from the third day post inoculation until day 30 th Dagli et al. The control group showed significant differences in tumor growth between days 7 th and 12 th. The growth rate of Ehrlich tumor in mice differs in the site of cnica and in the form. In the solid form occurs a proliferation peak after the 7 th day of implantation Silva et al.
There was a significant reduce in the arrabidasa growth in mice treated with AE and EE at 12 th day, different from control group Figure 1. Cancer is a disease of misguided cells that have high potential of excess proliferation without apparent relation to the physiological demand of the process.
It is the second largest cause of death in the world. The greatest recent impact of plant-derived drugs is observed in the area of antitumor research, where compounds such as taxol, vinblastine, vincristine, and camptothecin have dramatically improved the effectiveness of chemotherapy against some of the most dreaded cancers Rates, Hence, there is a great potential for the development of anticancer drugs from the essentially untapped reservoir of the plant kingdom.
A large number of plants possessing anticancer properties have been documented Gupta et al. Earlier studies carried out in our laboratory have shown potent cytotoxic activity of A. Based zrrabidaea this observation, in the present study, the aqueous and choca extracts were evaluated for its in vivo antitumor properties. arrabidaew
Evaluation of wound healing properties of Arrabidaea chica Verlot extract.
These results could indicate either a direct cytotoxic effect of A. The direct strong cytotoxic effect of ethanol extract was already demonstrated in the in vitro experiments against Jurkat and HL60 cell lines Ribeiro et al.
Preliminary phytochemical investigation of the ethanol extract of A.
The antifungal and trypanocidal activities of A. Flavonoids could also be involved in the trypanocidal activity, since plants synthesized them in response to microbial infection Barbosa et al. We believed that the antitumoral activity of A.
During the last decade, natural antioxidants, particularly phenolics, have been under very close scrutiny as potential therapeutic agents against a wide range of ailments including cancer, inflammatory diseases and also aging. The medicinal actions of phenolics is mostly ascribed to their antioxidant capacity, free xhica scavenging, chelation of redox active metal ions, modulation of gene expression and interaction with the cell signaling pathways Soobrattee et al.
Most chemotherapeutic agents induce collateral effects. Direct myelotoxicity is often observed in patients undergoing anticancer treatments.
Hence, the evaluation of toxicity can be accomplished by blood count evaluation Perez et al. None of the animals bearing Ehrlich Tumor control group and treated with A. The time of tumor development 12 days was probably not sufficient to cause exhaustion of the bone marrow.
Also, it might be inferred that the extracts tested did not produce toxic effects in animals. Another report showed that the hidroalcoholic extract ardabidaea A.
There were no differences in the total leukocytes between groups. However, the ethanol extract EE caused a significant increase in neutrophils count Table 3. Neutrophils play an important role in tumor angiogenesis releasing chemokines that cause endothelial cell invasion and vessel formation. Other studies showed that potent activation of infiltrating macrophages and granulocytes could lead to tumor destruction and tumor rejection Albini et al.
In our study we observed that the reduction in the size of the tumor in the EE group was accompanied by an increase in the number of neutrophils in blood. Also, this group presented a larger number of neutrophils in the histology of the tumor.
The treatment with AE, which also reduced tumor growth, did not alter the number of neutrophils in blood or differ from control group in concern to the morphology of the tumor. Tumor growth initiates a myriad of functional and phenotypic changes in macrophages and T-cells in association with alterations in cytokine synthesis and responsiveness Walker et al. Several types of tumor also express receptors for granulocyte-macrophage colony stimulating factors or produces and uses granulocyte-macrophage colony stimulating factors as an autocrine growth factor Fu et al.
Phagocytes, particularly macrophages and neutrophils, play a vital role in both the innate and the acquired immunity, exerting a key role in host defense against various infectious agents and tumor growth Natan et al, Animal model studies have shown that a variety of tumor cells can produce factors that impair inflammatory responses, thus allowing tumor growth in vivo.
Alternatively, the tumor cells can stimulate macrophage suppressor activities in host cells Elgert et al.